Derpharmachemica

Terminalia chebula, popularly known as Harad, black- or chebulic myrobalan contains hydrolysable tannins. This dominantly contains many phytochemicals viz., casuarinin, chebulanin, chebulagic acid, chebulinic acid corilagin, ellagic acid, gallic acid, neochebulinic acid, punicalagin, 1,2,3,4,6-penta-O-galloyl-β-D-glucose,1,6-di-o-galloyl-D-glucose,3,4,6-tri-o-glloyl-D-glucose, terchebulin. There are many medicinal investigations recording viz., antiulcer, antioxidant, anticarcinogenic, antimutagenic, radio protective, hepatoprotective, cardioprotective, cytoprotective, antidiabetic, renoprotective, antibacterial, antifungal, antiviral, antiprotozoal, anti-inflammatory, antiarthritic, antispasmodic, wound healing and anticonceptive, molluscidal, anthelmintic, anaphylactic, hypolipidemic, hypocholesterolemic. Also having chemopreventive potential and adaptogenic activities. This has purgative property, immunomodulatory, analgesic, antiallergic, neuroprotective, acetylcholine inhibition

A novel method for the estimation of Budesonide in human plasma by using LC-MS-MS and the analyte is budesonide and internal standard is levenorgestreol were extracted with the tertbutyl methyl ether: n-hexane (70:30, v/v) from human plasma. The chromatographic severance was attained of the peak using Agilent Zorbax Eclipse XDB-C8 , (100 mm × 4.6 mm, 3.5 µm) column with a run time is 2.5 min. Budesonide andlevenorgestreol were recorded at the total ion current of their relevant multiple reaction monitoring. The LC-MS-MS system composed an Agilent 1100 infinity combined with an AB Sciex Qtrap® 4000 thermo Finnigan TSQ quantum discovery triple quadruple mass spectrometer. All of the parameters must be validated like selectivity, accuracy, precision, linearity, lower limit of quantification, matrix effect, recovery reached the acceptance criteria under the following of ICH guidelines. Budesonide have checked the various stability studies like short term stability at 25°C, long term stab